Archives for April 2016

The Harsh Reality of Radioactive Labeling

Once commonly used, the hazards have now outweighed the benefits.

Oligonucleotides have been a major contributor in biological and forensic research for years now. Certain applications such as taking on the role of a sequence binding agent to reveal the present of a DNA sequence have provided researchers new information to work off of. In these applications, oligos are labeled for identification purposes. Some of these labels come from radioactive nuclei such as RNA triphosphates. When applied to the oligos, they can be directly added into nucleic acid probes via enzymatic methods like nick translations.

The Hazards of Radiolabeling

However, radioactive labeling does have its caveats. There are a variety of known hazards when handling any forms of radiation. In addition to this, radiolabelling requires there to be careful handling and disposing, which scientists need to closely be wary about. Although this method of labeling does have its advantages in that it provides high sensitivity for the oligo, or the modified oligo, but the expense of working with it makes it difficult to consistently utilize. Therefore, other labeling methods such as fluorescent labeling, colorimetric, and chemiluminescent have been becoming increasingly popular. In most application, radiolabelling isn’t even used anymore. But, every now and then, you’ll find that certain applications require the use of radioactive methods to achieve a breakthrough.

Labeling Requirements

In order for an oligo to be labeled, it must possess certain properties that scientists deem feasible. One of the most common ones would be that the label should be able to attach easily under mild conditions using any type of protocol – regardless of how simple or cheap it is. Another requirement is that the label can be detected at the lowest concentration, otherwise the findings would be inconclusive.


 

The Midland Certified Reagent Company carries all the supplies that you need to maintain the perfect oligo synthesis process. For more information, visit them online.

How to conduct a free online check?

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Article by Tenant Screening Services, LLC

Many people conduct background criminal checks on individuals to protect themselves as well as their loved ones. It is also a good idea to conduct a background criminal check on yourself. This can help you gather sensitive information that can be used by a potential employer, stalker or exes. Instant criminal background check online can be expensive and require a lot of information that you may not have. Here are a few tips on how to conduct a free background check online.

Target’s name – Use search engines and social networks such as Google, Facebook, LinkedIn, and Twitter. To narrow your search, try entering any other information you know about your target, for example if your target’s name is Jane Doe and she works at Mcdonalds, include Jane Doe; Mcdonalds in your search.

Searching Your Social Networks – Facebook is the best tool to provide information on your target. Since the information provided is self voluntary, it is a much used tool by many employers. If entering your target’s name and email address does not provide you with any results, this maybe because they have placed their profile on private and unsearchable. In this instance, you can do a site specific google search. This will bring up any public pages or groups they have commented on.

Finding your target’s phone number and address – To find your target’s phone number, you can use ZabaSearch. For mobile phone numbers a good web site is WhitePages.com. If you’re successful and locate a mobile number, it will also include an address for your target.

Tenant Screening Services, LLC provides tenant screening services including criminal background checks.